DOPA (3,4-dihydroxy-L-phenylalanine)

DOPA (3,4-dihydroxy-L-phenylalanine)

CAS Number59-92-7
Molecular FormulaC9H11NO4
Molecular Weight197.190
InChI KeyWTDRDQBEARUVNC-LURJTMIESA-N
LogP-2.39
Synonyms
  • L-Dopa
  • 3-Hydroxy-L-tyrosine
  • L-Tyrosine, 3-hydroxy-
  • 59-92-7
  • Levodopa
  • L-Tyrosine, 3-hydroxy-
  • (-)-3,4-Dihydroxyphenylalanine
  • (-)-Dopa
  • (2S)-2-Amino-3-(3,4-dihydroxyphenyl)propanoic acid
  • 3-(3,4-Dihydroxyphenyl)-L-alanine
  • 3,4-Dihydroxy-L-phenylalanine
  • 3,4-Dihydroxyphenyl-L-alanine
  • 3-Hydroxy-L-tyrosine
  • Alanine, 3-(3,4-dihydroxyphenyl)-, L-
  • Alphadopa
  • Bendopa
  • Cidandopa
  • Deadopa
  • Dihydroxy-L-phenylalanine
  • DOPA, L-
  • Dopaflex
  • Dopaidan
  • Dopalina
  • Doparkine
  • Dopasol
  • Dopaston
  • Dopaston SE
  • Dopastone
  • Dopastral
  • Dopicar
  • Eldopal
  • Eldopar
  • Eldopatec
  • Eurodopa
  • Helfo-dopa
  • Insulamina
  • L-(-)-Dopa
  • L-3-(3,4-Dihydroxyphenyl)alanine
  • L-4,5-Dihydroxyphenylalanine
  • Laradopa
  • Larodopa
  • L-β-(3,4-Dihydroxyphenyl)-α-alanine
  • Maipedopa
  • Pardopa
  • Syndopa
  • Veldopa
  • Weldopa
  • β-(3,4-Dihydroxyphenyl)alanine
  • β-(3,4-Dihydroxyphenyl)-L-alanine
  • β-(3,4-Dihydroxyphenyl)-α-L-alanine
  • Alanine, 3-(3,4-dihydroxyphenyl)-, (-)-
  • Biodopa
  • Brocadopa
  • Cerepap
  • L-(o-Dihydroxyphenyl)alanine
  • L-O-Dihydroxyphenylalanine
  • L-beta-(3,4-Dihydroxyphenyl)alanine
  • (-)-3-(3,4-Dihydroxyphenyl)-L-alanine
  • beta-(3,4-Dihydroxyphenyl)-alpha-alanine
  • beta-(3,4-Dihydroxyphenyl)-L-alanine
  • 3,4-Dihydroxyphenylalanine
  • Dopal-fher
  • EINECS 200-445-2
  • Helfo DOPA
  • L-o-Hydroxytyrosine
  • NSC 118381
  • Sobiodopa
  • Levodopum
  • UNII-46627O600J
  • (2S)-2-amino-3-(3,4-dihydroxyphenyl)propanoate
  • (2S)-2-amino-3-(3,4-dihydroxyphenyl)propionic acid
  • Dihydroxyphenylalanine
  • Dopal
  • Dopar
  • Doparl
  • Doprin
  • L-3-(3,4-Dihydroxyphenyl)-Alanine
  • L-4-5-Dihydroxyphenylalanine
  • L-Dihydroxyphenylalanine
  • L-b-(3,4-Dihydroxyphenyl)-a-alanine
  • L-beta-(3,4-Dihydroxyphenyl)-alpha-alanine
  • Ledopa
  • Levedopa
  • Levopa
  • Parda
  • Prodopa
  • b-(3,4-Dihydroxyphenyl)-L-alanine
  • b-(3,4-Dihydroxyphenyl)-a-L-alanine
  • b-(3,4-Dihydroxyphenyl)alanine
  • beta-(3,4-Dihydroxyphenyl)-alpha-L-alanine
  • beta-(3,4-dihydroxyphenyl)-L-alanine
  • beta-(3,4-dihydroxyphenyl)alanine
  • (2S)-2-ammonio-3-(3,4-dihydroxyphenyl)propanoate
  • L-dopa zwitterion
  • 23734-74-9
  • 25525-15-9
  • 34241-25-3
  • 72572-99-7
  • 72573-00-3
  • 88250-23-1
  • 90638-38-3

Applications:

UV-Vis Spectrum of L-DOPA

July 19, 2024
UV-Vis Spectrum of L-DOPA. Absorption Maxima: 198 nm, 280 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Generic Screening Method for Complex Mixtures on Primesep 200

October 15, 2015

Condition

Column Primesep 200, 4.6*150 mm 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 215 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline

 

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethylparaben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Neurotransmitters

November 25, 2011

The neurotransmitters dl-DOPA, creatinine, hydroxytryptophan (5-HTP), and serotonin are separated in less than 4 minutes on a short 50 mm Primesep 200 column. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Ion-pair reagents were not needed for retention of these polar, hydrophilic compounds, instead, a combination of ion-exchange and reversed-phase interactions were used.

Condition

Column Primesep 200, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 80/20%
Buffer TFA – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds dl-DOPA, Creatinine, Hydroxytryptophan (5-HTP), Serotonin

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Creatinine
DOPA (3,4-dihydroxy-L-phenylalanine)
Hydroxytryptophan
Serotonin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Amino Acids in HILIC and Cation-Exchange Modes

July 14, 2011

chr_306.gif

Amino acids can be retained and separated on a Primesep S2 column in HILIC/cation-exchange mode.

Condition

Column Primesep S2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Dopa, Tyrosine, Phenylalanine

 

Application Column

Primesep S2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Phenylalanine
Tyrosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Catecholamines on Primesep 100 Column with Phosphate Buffers

July 8, 2011

chr_284.gif

The catecholamine neurotransmitters are amino-acid derivatives of tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine and baseline are resolved on a Primesep 100 column with UV-transparent phosphate buffer. This method can be used for analysis of catecholamines and related impurities in various matrices. Peak order and retention time can be changed by changing the amount of ACN, buffer concentration and buffer pH. Various buffers can be used to accommodate desired detection technique. Primesep 100 is a reversed-phase cation-exchange mixed-mode column that can be used for analysis of polar neutral, polar ionizable, polar zwitter-ionic, hydrophobic neutral, and hydrophobic ionic compounds in the same run. Column can be operated in reverse-phase, cation-exchange, anion-exclusion, HILIC and mixed-modes depending on the mobile phase selection and nature of analytes. Column is compatible with LC/MS and does not require use of ion-pairing reagents.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Na2HPO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Tyrosine, DOPA, Phenylalanine, Norepinephrine, Epinephrine, Dopamine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Dopamine
Epinephrine
Norepinephrine
Phenylalanine
Tyrosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Serotonin, Dopamine, and Related Compounds

August 22, 2008

Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of the catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. The method is very sensitive to amount of ACN, buffer and buffer pH. The retention time changes with variation of the main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by a combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).

Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Homovanillic acid, Dihydroxyphenyl acetic acid, Serotonin, Dopamine, Epinephrine, Hydroxytryptophan, DOPA

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Epinephrine
Homovanillic Acid
Hydroxytryptophan
Norepinephrine
Serotonin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Polar and Hydrophobic Drugs on Obelisc R

March 3, 2007

Condition

Column Obelisc R, 4.6 x 150 mm, 5 µm, 100 A
Mobile Phase MeCN/H2O – 40/60%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 220 nm

 

Description

Class of Compounds
Drug, Acid, Preservative,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Propyl paraben,  Dopamine, DOPA

 

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Dopamine
Propylparaben

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Compounds of Catecholamine Pathway

March 3, 2006

The catecholamine neurotransmitters are amino-acid derivatives of tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine and baseline are resolved on a
Primesep 200 column with UV-transparent phosphate buffer. This method can be used for the analysis of catecholamines and related impurities in various matrices. The peak order and retention time can be changed by changing the amount of ACN, buffer concentration and buffer pH. Various buffers can be used to accommodate the desired detection technique. Primesep 200 is a reversed-phase cation-exchange mixed-mode column that can be used for analysis of polar neutral, polar ionizable, polar zwitterionic, hydrophobic neutral, and hydrophobic ionic compounds in the same run. Column can be operated in reverse-phase, cation-exchange, anion-exclusion, HILIC and mixed-modes depending on the mobile phase selection and the nature of the analytes. The column is compatible with LC/MS and does not require the use of ion-pairing reagents.

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, AmFm
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Tyrosine, DOPA, Phenylalanine, Norepinephrine, Epinephrine, Dopamine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Dopamine
Epinephrine
Phenylalanine
Tyrosine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (1)

January 13, 2005


Amino acids are the building blocks of proteins and are widely used as supplements.  Amino acids can exist in acidic, basic or zwitterionic form depending on the pH of their environment (mobile phase in this case).  Primesep 200 is a reverse-phase column with embedded weak acidic ion-pairing groups which allows for retention and separation of amino acids without requiring a buffer making it ideal for use with UV detection, mass spectrometry (MS), evaporative light scattering detection (ELSD).

Condition

Column Primesep 200, 3.0×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer No
Flow Rate 0.5 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Dopa, Phenylalanine, Tyrosine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Phenylalanine
Tyrosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Polar and Hydrophobic Drugs on Obelisc N Cloumn

June 11, 2004

Condition

Column Obelisc N, 4.6 x 150 mm, 5 µm, 100 A
Mobile Phase MeCN/H2O – 80/20%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 220 nm

 

Description

Class of Compounds
Drug, Acid, Preservative,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Propyl paraben,  Dopamine, DOPA

 

Application Column

Obelisc N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Dopamine
Propylparaben
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Analysis of the Catecholamine Pathway

January 17, 2004

Primesep 200 separates catecholamines in the catecholamine pathway in 10 minutes. Phenylalanine, tyrosine, DOPA, dopamine, norepinephrine, and epinephrine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.

Condition

Column Primesep 200, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer TFA – 0.1%
Flow Rate 0.5 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Tyrosine, DOPA, Phenylalanine, Norepinephrine, Epinephrine, Dopamine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)
Dopamine
Epinephrine
Phenylalanine
Tyrosine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.