HPLC Analysis of the Catecholamine Pathway

Primesep 200 separates catecholamines in the catecholamine pathway in 10 minutes. Phenylalanine, tyrosine, DOPA, dopamine, norepinephrine, and epinephrine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.


Column Primesep 200, 3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer TFA - 0.1%
Flow Rate 0.5 ml/min
Detection UV, 210 nm


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Tyrosine, DOPA, Phenylalanine, Norepinephrine, Epinephrine, Dopamine
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Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)

Application Detection:
UV Detection