Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (1)

Amino acids are the building blocks of proteins and are widely used as supplements.  Amino acids can exist in acidic, basic or zwitterionic form depending on the pH of their environment (mobile phase in this case).  Primesep 200 is a reverse-phase column with embedded weak acidic ion-pairing groups which allows for retention and separation of amino acids without requiring a buffer making it ideal for use with UV detection, mass spectrometry (MS), evaporative light scattering detection (ELSD).


Column Primesep 200, 3.0x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer No
Flow Rate 0.5 ml/min
Detection UV, 210 nm


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Dopa, Phenylalanine, Tyrosine
Application Analytes:
DOPA (3,4-dihydroxy-L-phenylalanine)