
| CAS Number | 5036-02-2 |
|---|---|
| Molecular Formula | C11H12N2S |
| Molecular Weight | 204.29 |
| InChI Key | HLFSDGLLUJUHTE-UHFFFAOYSA-N |
| LogP | 1.8 |
| Synonyms |
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Applications:
HPLC MS Method for Analysis of Tetramisole on Primesep 200 Column
August 25, 2026
HPLC Method for Analysis of (+-)-Tetramisole on Primesep NC by SIELC Technologies

Tetramisole is a medication with the chemical formula C11H12N2S. It is primarily used to treat and get rid of parasitic worms in animals. It works through inhibiting alkalinephosphates, which freezes muscles of the worm. You can find detailed UV spectra of (+-)-Tetramisole and information about its various lambda maxima by visiting the following link.
(+-)-Tetramisole can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and ammonium formate. Detection is performed using MS.
Condition
| Column | Primesep 200, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O -40/60% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | ESI-SIM: [M-H]– m/z 205 |
Description
| Class of Compounds | Drugs |
| Analyzing Compounds | (+-)-Tetramisole |
Application Column
Primesep 200
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Tetramisole
February 24, 2026
Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze (+-)-Tetramisole check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
