Suprofen

Suprofen structural formula

CAS Number40828-46-4
Molecular FormulaC14H12O3S
Molecular Weight260.310
InChI KeyMDKGKXOCJGEUJW-UHFFFAOYSA-N
LogP2.77
Synonyms
  • Suprofen
  • 2-[4-(Thiophene-2-carbonyl)phenyl]propanoic acid
  • Benzeneacetic acid, alpha-methyl-4-(2-thienylcarbonyl)-
  • 40828-46-4
  • Benzeneacetic acid, ?-methyl-4-(2-thienylcarbonyl)-
  • (.+-.)-Suprofen
  • 2-[4-(Thiophene-2-carbonyl)phenyl]propanoic acid
  • Masterfen
  • NSC 303611
  • p-(2-Thenoyl)hydratropic acid
  • Profenal
  • Profenol
  • Racemic suprofen
  • Srendam
  • Sulproltin
  • Supranol
  • Suprocil
  • suprofene
  • Sutoprofen
  • Topalgic
  • EINECS 255-096-9
  • Maldocil
  • p-2-Thenoylhydratropic acid
  • (+-)-2-(p-(2-Thenoyl)phenyl)propionic acid
  • Suprofenum
  • UNII-988GU2F9PE
  • 2-(4-(2-Thenoyl)phenyl)propionsaeure
  • 4-(2-Thenoyl)hydratropsaeure
  • alpha-methyl-4-(2-thienylcarbonyl)benzeneacetic acid
  • p-2-thenoylhydratropic acid

Applications:

HPLC Chiral Separation of Suprofen Enantiomers on Cyralis AM Column

July 29, 2026

HPLC Chiral Method for Analysis of Suprofen Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Suprofen.

Suprofen is a non-steroidal anti-inflammatory drug (NSAID) with the chemical formula C14H12O3S. It is no longer in use in the united states due to real effects. Previously, it was used to prevent miosis during and after ophthalmic surgery.

This application describes a normal-phase chiral HPLC method for the separation of Suprofen enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Suprofen can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 150 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/IPA/TFA
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution1.78

Description

Class of CompoundsDrug
Analyzing CompoundsSuprofen

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Suprofen
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Suprofen on Newcrom R1 HPLC column

February 16, 2018
Separation of Suprofen on Newcrom C18 HPLC column

Suprofen can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Suprofen
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.