HPLC Chiral Separation of Suprofen Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Suprofen Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Suprofen.

Suprofen is a non-steroidal anti-inflammatory drug (NSAID) with the chemical formula C14H12O3S. It is no longer in use in the united states due to real effects. Previously, it was used to prevent miosis during and after ophthalmic surgery.

This application describes a normal-phase chiral HPLC method for the separation of Suprofen enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Suprofen can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 150 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/IPA/TFA
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution1.78

Description

Class of CompoundsDrug
Analyzing CompoundsSuprofen

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Suprofen
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.