Spectinomycin

Spectinomycin structural formula

CAS Number1695-77-8
Molecular FormulaC14H24N2O7
Molecular Weight332.353
InChI KeyUNFWWIHTNXNPBV-WXKVUWSESA-N
LogP-0.376
Synonyms
  • Spectinomycin
  • (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-4a,7,9-Trihydroxy-2-methyl-6,8-bis(methylamino)decahydro-4H-pyrano[2,3-b][1,4]benzodioxin-4-one
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-
  • 1695-77-8
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, [2R-(2?,4a?,5a?,6?,7?,8?,9?,9a?,10a?)]-
  • Actinospectacin
  • Antibiotic 2233wp
  • Benzeneacetamide, N-ethyl-?-(hydroxymethyl)-N(4-pyridinylmethyl)-
  • Espectinomicina
  • Spectacin
  • Spectam
  • spectinomycine
  • Togamycin
  • Trobicin
  • BRN 2171701
  • EINECS 216-911-3
  • Actinospectacina
  • Spectinomicina
  • Spectinomycinum
  • UNII-93AKI1U6QF

Applications:

HPLC Determination of Spectinomycin on Obelisc R Column

November 25, 2020

HPLC Method for Spectinomycin on Obelisc R by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Spectinomycin.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

Spectinomycin is an antibiotic with the chemical formula C14H24N2O7. It is typically used to treat gonorrhea infections through injection. It works by binding itself to the 30S ribosomal subunit which leads to prevention of movement of mRNA and tRNA molecules during protein synthesis. It can be found under the brand name Trobicin.

Spectinomycin can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Acetate buffer. Detection is performed using CAD.

Condition
Column Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN -30%,
Buffer Ammonium Acetate pH 3.5 – 50 mM
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
  Antibiotics, Drug
Analyzing Compounds Spectinomycin

 

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Spectinomycin
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Spectinomycin on Newcrom R1 HPLC column

May 16, 2018
Separation of Spectinomycin on Newcrom R1 HPLC column

Spectinomycin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Spectinomycin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.