Spectinomycin

Spectinomycin structural formula

CAS Number1695-77-8
Molecular FormulaC14H24N2O7
Molecular Weight332.353
InChI KeyUNFWWIHTNXNPBV-WXKVUWSESA-N
LogP-0.376
Synonyms
  • Spectinomycin
  • (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-4a,7,9-Trihydroxy-2-methyl-6,8-bis(methylamino)decahydro-4H-pyrano[2,3-b][1,4]benzodioxin-4-one
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-
  • 1695-77-8
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, (2R,4aR,5aR,6S,7S,8R,9S,9aR,10aS)-
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-
  • 4H-Pyrano[2,3-b][1,4]benzodioxin-4-one, decahydro-4a,7,9-trihydroxy-2-methyl-6,8-bis(methylamino)-, [2R-(2?,4a?,5a?,6?,7?,8?,9?,9a?,10a?)]-
  • Actinospectacin
  • Antibiotic 2233wp
  • Benzeneacetamide, N-ethyl-?-(hydroxymethyl)-N(4-pyridinylmethyl)-
  • Espectinomicina
  • Spectacin
  • Spectam
  • spectinomycine
  • Togamycin
  • Trobicin
  • BRN 2171701
  • EINECS 216-911-3
  • Actinospectacina
  • Spectinomicina
  • Spectinomycinum
  • UNII-93AKI1U6QF

Applications:

HPLC Determination of Spectinomycin on Obelisc R Column

November 25, 2020


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Condition
Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN -30%,
Buffer Ammonium Acetate pH 3.5 – 50 mM
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
  Antibiotics, Drug
Analyzing Compounds Spectinomycin

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Spectinomycin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Spectinomycin on Newcrom R1 HPLC column

May 16, 2018
Separation of Spectinomycin on Newcrom R1 HPLC column

Spectinomycin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Spectinomycin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.