| CAS Number | 79-09-4 |
|---|---|
| Molecular Formula | C3H6O2 |
| Molecular Weight | 74.080 |
| InChI Key | XBDQKXXYIPTUBI-UHFFFAOYSA-N |
| LogP | 0.33 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Propionic Acid
January 5, 2026
If you are looking for optimized HPLC method to analyze Propionic acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Simultaneous Determination of Formamide, Propionic Acid and Formic Acid on Newcrom BH Column
August 24, 2022
HPLC Method for Formamide, Propionic acid, Formic acid on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Formamide, Propionic acid, Formic acid.
Formamide is a popular industrial solvent with the chemical formula CH3NO. It has a wide variety of applications, including in the manufacturing of drugs, herbicides, pesticides, as a paper and fiber softener, and as a solvent for a plethora of ionic compounds. Historically, it was produced by treating formic acid and ammonia.
Propionic acid is a popular antifungal and antibacterial compound with C3H6O2. It is an oily liquid with an unpleasant smell. Primarily, the acid is used in food production as a preservative.
Formic Acid is the simplest carboxylic acid with the chemical formula CH2O2. It is naturally found in insects, weeds, fruits, and vegetables. It is used by insects as a method of self-defense. In agriculture, it is used as a preservative and antibacterial agent. In chromatography, it is used as a volatile pH modifier. It is used significantly in the tanning of leather, dyeing and finishing of textiles, and production of rubber.
Formamide, Propionic acid, Formic acid can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
| Column | Newcrom BH, 4.6 x 250 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 10 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Formamide, Propionic acid, Formic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Formic acid
Propionic acid
Separation of Propionic acid on Newcrom R1 HPLC column
May 16, 2018
Propionic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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