Procarbazine

Procarbazine structural formula

CAS Number671-16-9
Molecular FormulaC12H19N3O
Molecular Weight221.305
InChI KeyCPTBDICYNRMXFX-UHFFFAOYSA-N
LogP0.06
Synonyms
  • Procarbazine
  • 4-[(2-Methylhydrazinyl)methyl]-N-(propan-2-yl)benzamide
  • Benzamide, N-(1-methylethyl)-4-[(2-methylhydrazinyl)methyl]-
  • 671-16-9
  • Benzamide, N-(1-methylethyl)-4-[(2-methylhydrazino)methyl]-
  • 1-Methyl-2-[p-(isopropylcarbamoyl)benzyl]hydrazine
  • 4-[(2-Methylhydrazino)methyl]-N-isopropylbenzamide
  • Benzamide, N-(1-methylethyl)-4-[(2-methylhydrazinyl)methyl]-
  • Ibenzmethyzine
  • N-Isopropyl-4-(2-methylhydrazinomethyl) benzamide
  • N-Isopropyl-?-(2-methylhydrazino)-p-toluamide
  • p-(2-Methylhydrazinomethyl)-N-isopropylbenzamide
  • Procarbazin
  • procarbazina
  • p-Toluamide, N-isopropyl-?-(2-methylhydrazino)-
  • BRN 0958270
  • EINECS 211-582-2
  • N-(1-Methylethyl)-4-((2-methylhydrazino)methyl)benzamide
  • p-(N'-Methylhydrazinomethyl)-N-isopropylbenzamide
  • 1-Methyl-2-(p-(isopropylcarbamoyl)benzyl)hydrazine
  • Natulan
  • Procarbazinum
  • UNII-35S93Y190K
  • 2-(p-Isopropylcarbamoylbenzyl)-1-methylhydrazine
  • 4-((2-Methylhydrazino)methyl)-N-isopropylbenzamide
  • N-(1-Methylethyl)-4-((2-methylhydrazino)methyl)benzamide
  • N-4-Isopropylcarbamoylbenzyl-N'-methylhydrazine
  • N-Isopropyl-alpha-(2-methylhydrazino)-p-toluamide
  • N-Isopropyl-p-(2-methylhydrazinomethyl)-benzamide
  • N-isopropyl-4-[(2-methylhydrazino)methyl]benzamide

Applications:

HPLC UV Method for Analysis of Procarbazine Hydrochloride on Primesep 100 Column

October 27, 2026

HPLC Method for Procarbazine hydrochloride, Procarbazine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Procarbazine hydrochloride, Procarbazine on Primesep 100 Column by SIELC Technologies

Procarbazine is a chemotherapy medication with the formula C12H19N3O. It is used primarily in treatment of Hodgkin lymphoma and brain cancers. Itison the World Health Organization’s List of Essential Medicines. In treatment of Hodgkin’s lymphoma, it is typically delivered as a part of the BEACOPP regiment, being the last “P” in the acronym. It is used in other combinations, usually as a part of PCV, when treating brain cancer. You can find detailed UV spectra of Procarbazine hydrochloride, Procarbazine and information about its various lambda maxima by visiting the following link.

Procarbazine hydrochloride, Procarbazine can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and H2SO4 as a buffer. Detection is carried out using UV

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50%
BufferH2SO4  – 0.1%
Flow Rate1 ml/min
DetectionUV 230 nm
LOD*6 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of CompoundsMedication
Analyzing CompoundsProcarbazine hydrochloride, Procarbazine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Procarbazine
Procarbazine hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Procarbazine on Newcrom R1 HPLC column

February 16, 2018
Separation of Procarbazine on Newcrom C18 HPLC column

Procarbazine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Procarbazine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.