Olsalazine

Olsalazine structural formula

CAS Number15722-48-2
Molecular FormulaC14H10N2O6
Molecular Weight302.243
InChI KeyQQBDLJCYGRGAKP-UHFFFAOYSA-N
LogP3.73
Synonyms
  • Olsalazine
  • 3,3'-Diazenediylbis(6-hydroxybenzoic acid)
  • Benzoic acid, 3,3'-(1,2-diazenediyl)bis[6-hydroxy-
  • 15722-48-2
  • Benzoic acid, 3,3'-azobis[6-hydroxy-
  • 3,3'-Azobis(6-hydroxybenzoic acid)
  • 3,3'-Dicarboxy-4,4'-dihydroxyazobenzene
  • 5,5'-Azobis(salicylic acid)
  • 5,5'-Azodisalicylic acid
  • Azodisal
  • Benzoic acid, 3,3'-(1,2-diazenediyl)bis[6-hydroxy-
  • C.I. Mordant Yellow 5
  • Salicylic acid, 5,5'-azodi-
  • Dipentium
  • UNII-ULS5I8J03O
  • Olsalazina
  • Olsalazinum

Applications:

Uv-Vis Spectrum of Olsalazine

February 17, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Olsalazine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Olsalazine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Olsalazine on Newcrom R1 Column

July 12, 2022

HPLC Method for Olsalazine on Newcrom R1 by SIELC Technologies

HPLC Method for Analysis of Olsalazine on Newcrom R1 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Olsalazine.

Olsalazine is a prodrug of mesalazine with the chemical formula C14H10N2O6. It has anti-inflammatory effects when it goes through colonic breakdown.  You can find detailed UV spectra of Olsalazine and information about its various lambda maxima by visiting the following link.

Condition
Column Newcrom R1, 2.1 x 100 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN – 50%
Buffer H2SO4 – 0.2
Flow Rate 1.0 ml/min
Detection UV 224 nm

Description
Class of Compounds
 Drug
Analyzing Compounds Olsalazine

Application Column

Newcrom R1

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Olsalazine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Olsalazine on Newcrom R1 HPLC column

May 16, 2018
Separation of Olsalazine on Newcrom R1 HPLC column

Olsalazine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Olsalazine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.