| CAS Number | 846-49-1 |
|---|---|
| Molecular Formula | C15H10Cl2N2O2 |
| Molecular Weight | 321.161 |
| InChI Key | DIWRORZWFLOCLC-UHFFFAOYSA-N |
| LogP | 2.39 |
| Synonyms |
|
Applications:
UV-Vis Spectrum of Lorazepam
July 11, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Lorazepam Injection on Newcrom R1 Column
July 22, 2021
Separation type: Liquid Chromatography Reverse Phase.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Lorazepam.
Lorazepam is a short-acting benzodiazepine medication commonly used as a sedative and anxiolytic of choice in the inpatient setting owing to its fast onset of action when administered intravenously. Lorazepam is one of the few sedative-hypnotics with a relatively clean side effect profile. Lorazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 275 nm.
| Column | Newcrom R1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 35% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | 275 nm |
| Class of Compounds | Hydrophobic, Drug |
| Analyzing Compounds | Benzyl Alchohol, Lorazepam |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsLorazepam
Separation of Lorazepam on Newcrom R1 HPLC column
February 19, 2018
Lorazepam can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
