HPLC Method for Lorazepam Injection on Newcrom R1 Column

Separation type: Liquid Chromatography Reverse Phase.

View on hplc.cloud


High Performance Liquid Chromatography (HPLC) Method for Analysis of  Lorazepam.

Lorazepam is a short-acting benzodiazepine medication commonly used as a sedative and anxiolytic of choice in the inpatient setting owing to its fast onset of action when administered intravenously. Lorazepam is one of the few sedative-hypnotics with a relatively clean side effect profile. Lorazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 275 nm.


Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 35%
Buffer No
Flow Rate 1.0 ml/min
Detection 275 nm


Class of Compounds Hydrophobic, Drug
Analyzing Compounds Benzyl Alchohol, Lorazepam


Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Benzyl alcohol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.