HPLC Chiral Separation of Lorazepam Enantiomers on Cyralis CM Column

HPLC Method for Analysis of Lorazepam on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Lorazepam.

Lorazepam is a short-acting benzodiazepine medication commonly used as a sedative and anxiolytic of choice in the inpatient setting owing to its fast onset of action when administered intravenously. Lorazepam is one of the few sedative-hypnotics with a relatively clean side effect profile. It has the chemical formula C15H10Cl2N2O2.

You can find detailed UV spectra of Lorazepam and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Lorazepam enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Lorazepam can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/Ethanol/DEA – 80/20/0.1%
Flow Rate0.5 mL/min
DetectionUV 250 nm
Resolution2.75

Description

Class of CompoundsMedication
Analyzing CompoundsLorazepam

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Lorazepam
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.