Separation type: Liquid Chromatography Mixed-mode
View on hplc.cloud
High Performance Liquid Chromatography (HPLC) Method for Analysis of Enalapril and Lisinopril
Enalapril and lisinopril are medications that work on the kidneys to help lower blood pressure. Lisinopril can be used for heart failure and for people who’ve had a heart attack. Enalapril can also be used for heart failure but not heart attacks. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, enalapril and lisinopril, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a sulfuric acid (H2SO4) buffer. UV detection at 210 nm.
|Column||Newcrom AH, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 50/50%|
|Buffer||H2SO4 - 0.5%|
|Flow Rate||1.0 ml/min|
|Detection||UV, 210 nm|
|Class of Compounds||Drug|
|Analyzing Compounds||Enalapril, Lisinopril, Maleic Acid|
Lisinopril can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.