HPLC Separation of Enalapril and Lisinopril on Newcrom AH Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Enalapril and Lisinopril
Enalapril and lisinopril are medications that work on the kidneys to help lower blood pressure. Lisinopril can be used for heart failure and for people who’ve had a heart attack. Enalapril can also be used for heart failure but not heart attacks. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, enalapril and lisinopril, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a sulfuric acid (H2SO4) buffer. UV detection at 210 nm.


Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer  H2SO4 - 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm


Class of Compounds Drug
Analyzing Compounds Enalapril,  Lisinopril, Maleic Acid
Application Analytes:
Lisinopril dihydrate