HPLC Separation of Enalapril and Lisinopril on Newcrom AH Column

HPLC Method for Enalapril, Lisinopril, Lisinopril dihydrate on Newcrom AH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Enalapril and Lisinopril

Enalapril is an ACE inhibitor medication with the chemical formula C20H28N2O5. It is used to treat hypertension, symptomatic heart failure, and asymptomatic left ventricular dysfunction. It also exhibited the ability to reduce the progression of kidney disease. Generic versions of it began their production in 2000.

Lisinopril is a medications that work on the kidneys to help lower blood pressure. It can be used for heart failure and for people who’ve had a heart attack. Like Enalapril, it is an ACE inhibitor. It has the chemical formula C21H31N3O5.

By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, enalapril and lisinopril, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a sulfuric acid (H2SO4) buffer. UV detection at 210 nm.

Condition
Column Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer  H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug
Analyzing Compounds Enalapril, Lisinopril, Lisinopril dihydrate

 

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Enalapril
Lisinopril
Lisinopril dihydrate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.