Enalapril

Enalapril structural formula

CAS Number75847-73-3
Molecular FormulaC20H28N2O5
Molecular Weight376.454
InChI KeyGBXSMTUPTTWBMN-XIRDDKMYSA-N
LogP0.07
Synonyms
  • Enalapril
  • N-[(2S)-1-Ethoxy-1-oxo-4-phenylbutan-2-yl]-L-alanyl-L-proline
  • L-Proline, N-[(1S)-1-(ethoxycarbonyl)-3-phenylpropyl]-L-alanyl-
  • 75847-73-3
  • L-Proline, N-[(1S)-1-(ethoxycarbonyl)-3-phenylpropyl]-L-alanyl-
  • (S)-1-(N-(1-(Ethoxycarbonyl)-3-phenylpropyl)-L-alanyl)-L-proline
  • Bonuten
  • Enalapril Richet
  • Gadopril
  • Enalaprila
  • Enalaprilum
  • UNII-69PN84IO1A
  • 1-(N-((S)-1-carboxy-3-phenylpropyl)-L-alanyl)-L-proline 1'-ethyl ester

Applications:

HPLC Separation of Enalapril and Lisinopril on Newcrom AH Column

June 3, 2021

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method for Analysis of Enalapril and Lisinopril


Enalapril and lisinopril are medications that work on the kidneys to help lower blood pressure. Lisinopril can be used for heart failure and for people who’ve had a heart attack. Enalapril can also be used for heart failure but not heart attacks. By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, enalapril and lisinopril, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a sulfuric acid (H2SO4) buffer. UV detection at 210 nm.





Condition
Column Newcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer  H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug
Analyzing Compounds Enalapril,  Lisinopril, Maleic Acid

 

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Enalapril
Lisinopril
Lisinopril dihydrate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Enalapril on Newcrom R1 HPLC column

February 16, 2018
Separation of Enalapril on Newcrom C18 HPLC column

Enalapril is a drug used to treat high blood pressure, diabetic kidney disease, and heart failure. It works by inhibiting angiotensin II, a blood pressure raiser. Enalapril can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Enalapril
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.