HPLC UV Method for Analysis of Polycyclic Aromatic Compounds (PACs) on Chromni Column

HPLC Method for Pyrene, Benzo[e]pyrene, Benz(a)anthracene, Dibenzothiophene on Chromni by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyrene, Benzo[e]pyrene, Benz(a)anthracene, Dibenzothiophene.

Pyrene is a polycyclic aromatic hydrocarbon with the chemical formula C16H10. It is formed during incomplete combustion of organic compounds. It is one of the most studied organic molecules in the world when it comes to its photophysical properties. Its fluorescence emission spectrum is sensitive to the polarity of the solvent. You can find detailed UV spectra of Pyrene and information about its various lambda maxima by visiting the following link.

Benz(a)anthracene is a polycyclic aromatic hydrocarbon with the chemical formula C18H12. It is a carcinogen that is produced during incomplete combustion of organic matter. You can find detailed UV spectra of Benz(a)anthracene and information about its various lambda maxima by visiting the following link.

Benzo(e)pyrene is a polycyclic aromatic hydrocarbon with the chemical formula C20H12. You can find detailed UV spectra of Pyrene, Benzo[e]pyrene, Benz(a)anthracene, Dibenzothiophene and information about its various lambda maxima by visiting the following link.

Dibenzothiophene, also written as DBT and diphenylene sulfide, is an organosulfuric compound with the chemical formula C12H8S. It is primarily found as a problematic impurity in petroleum. DBT is prepared by the reaction of biphenyl and sulfur dichloride when aluminum chloride is present. You can find detailed UV spectra of Pyrene, Benzo[e]pyrene, Benz(a)anthracene, Dibenzothiophene and information about its various lambda maxima by visiting the following link.

Pyrene, Benzo[e]pyrene, Benz(a)anthracene, Dibenzothiophene can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeOH – 100%
BufferNone
Flow Rate1.0 mL/min
DetectionUV 264 nm
Class of Compounds
Polycyclic Aromatic Carbon
Analyzing CompoundsPyrene, Benzo[e]pyrene, Benz(a)anthracene, Dibenzothiophene

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benz(a)anthracene
Benzo[e]pyrene
Dibenzothiophene
Pyrene

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.