HPLC Separation of EDTA, Oxymetazoline and Benzyl Alcohol in Nasal Spray

HPLC Method for EDTA (Ethylenediaminetetraacetic Acid), Oxymetazoline, Benzyl alcohol, Oxymetazoline hydrochloride on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol

Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN – 2- 70%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description
Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA (Ethylenediaminetetraacetic Acid), Oxymetazoline, Benzyl alcohol, Oxymetazoline hydrochloride

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzyl alcohol
EDTA (Ethylenediaminetetraacetic Acid)
Oxymetazoline
Oxymetazoline hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.