HPLC Method for Analysis of Dicarbon Carboxylic Acids on Newcrom BH Column

HPLC Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid on Newcrom BH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

1. Glyoxylic Acid (CHO-COOH): This is the smallest α-keto acid. It is a yellowish solid that occurs naturally and is useful industrially. It participates in many biochemical reactions. Its reduced form, D-lactic acid, can be found in various microorganisms.
2. Glycolic Acid (HOCH2COOH): This is the smallest α-hydroxy acid (AHA). It’s derived from sugar cane and is a colorless, odorless, and hygroscopic crystalline solid. It is used in various skin-care products because of its capability to penetrate skin.
3. Acetic Acid (CH3COOH): It is a simple carboxylic acid. It’s an important chemical reagent and industrial chemical, primarily used in the production of cellulose acetate for photographic film and polyvinyl acetate for wood glue, as well as synthetic fibers and fabrics. It gives vinegar its sour taste and pungent smell.
4. Oxalic Acid (C2H2O4): This is a colorless crystalline solid that forms a colorless solution in water. It occurs naturally in many foods, but too much can lead to kidney stones. It’s used in many different industrial processes, including rust removal, bleaching, and as a reducing agent in developing photographic film.

These four acids be separate and analyzed on a reverse-phase Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A column with a mobile phase consisting of water and perchloric acid as a buffer modifier. This analysis method can be UV detected at 200 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid

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