HPLC Method for Analysis of Benzocaine on Primesep 200 Column

HPLC Method for Benzocaine on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzocaine

Benzocaine is a local anesthetic with the chemical formula C9H11NO2. It is primarily used to treat pain in the mouth, threat, and ears. On occasion it is also used on sunburns and hemorrhoids. It is also commonly used as a ingredient ibn other medications. You can find detailed UV spectra of Benzocaine and information about its various lambda maxima by visiting the following link.

Benzocaine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 200, 3.2 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80 %
BufferH2SO4 – 0.1%
Flow Rate0.5 ml/min
DetectionUV
Limit of Detection*10 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds
 Amino Ester
Analyzing CompoundsBenzocaine

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzocaine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.