| CAS Number | 94-09-7 |
|---|---|
| Molecular Formula | C9H11NO2 |
| Molecular Weight | 165.193 |
| InChI Key | BLFLLBZGZJTVJG-UHFFFAOYSA-N |
| LogP | 1.86 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Benzocaine on Primesep 200 Column
April 13, 2026
HPLC Method for Benzocaine on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzocaine
Benzocaine is a local anesthetic with the chemical formula C9H11NO2. It is primarily used to treat pain in the mouth, threat, and ears. On occasion it is also used on sunburns and hemorrhoids. It is also commonly used as a ingredient ibn other medications. You can find detailed UV spectra of Benzocaine and information about its various lambda maxima by visiting the following link.
Benzocaine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid as a buffer. Detection is performed using UV.
| Column | Primesep 200, 3.2 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80 % |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV |
| Limit of Detection* | 10 ppb |
| Class of Compounds | Amino Ester |
| Analyzing Compounds | Benzocaine |
Application Column
Primesep 200
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of Benzocaine
April 10, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Benzocaine, Benzocaine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.
Benzocaine
Separation of Benzocaine on Newcrom R1 HPLC column
May 16, 2018
Benzocaine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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