HPLC Separation of Antiviral Drugs on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode





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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Oseltamivir, Valacyclovir


Unlike antibiotic drugs, which actively destroy their targets, antiviral drugs generally prevent the reproduction of their viral targets. Acyclovir, Valacyclovir, and Oseltamivir are 3 antiviral drugs commonly prescribed to treat viral infections. Acyclovir and Valacyclovir are used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. They can also be used to treat chickenpox in children. Oseltamivir is generally used to help treat (or if taken early enough, prevent) influenza (the flu) infections.
Acyclovir, Valacyclovir, and Oseltamivir can be retained on a Primesep 100 mixed-mode column with great peak shape using acetonitrile (ACN), water and a gradient of sulphuric acid (H2SO4) as a buffer. This method can be UV detected at 200 nm with very high resolution.



Condition
Column Primesep 100, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer Gradient H2SO4 – 0.05- 0.8%, 10 min
Flow Rate 0.5 ml/min
Detection UV, 255 nm

 

Description
Class of Compounds
 Drug
Analyzing Compounds Acyclovir, Valacyclovir, Oseltamivir

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Acyclovir
Oseltamivir
Valacyclovir
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.