HPLC Method for Labetalol on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Labetalol
Labetalol is a medication used to treat high blood pressure (hypertension). It is a combined alpha- and beta-blocker, which means it works by blocking both alpha and beta receptors in the body.
Labetalol can be retained, and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Formic Acid as a buffer. Detection is achieved using UV at 300 nm
You can find detailed UV spectra of Labetalol and information about its various lambda maxima by visiting the following link.
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 300 nm, SIM + 329 |
| Class of Compounds | Beta-blocker |
| Analyzing Compounds | Labetalol |
Application Column
Primesep 200
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
LabetalolApplication Detection:
UV DetectionLC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
