| CAS Number | 36894-69-6 |
|---|---|
| Molecular Formula | C19H24N2O3 |
| Molecular Weight | 328.412 |
| InChI Key | SGUAFYQXFOLMHL-UHFFFAOYSA-N |
| LogP | 3.09 |
| Synonyms |
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Applications:
HPLC MS Method for Analysis of Labetalol in Pharmaceutical Dosage Form on Primesep 200 Column
August 8, 2024
HPLC Method for Labetalol on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Labetalol
Labetalol is a medication used to treat high blood pressure (hypertension). It is a combined alpha- and beta-blocker, which means it works by blocking both alpha and beta receptors in the body.
Labetalol can be retained, and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Formic Acid as a buffer. Detection is achieved using UV at 300 nm
You can find detailed UV spectra of Labetalol and information about its various lambda maxima by visiting the following link.
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 300 nm, SIM + 329 |
| Class of Compounds | Beta-blocker |
| Analyzing Compounds | Labetalol |
Application Column
Primesep 200
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
LC MS Detection
HPLC Method for Analysis of Labetalol (stereoisomers) in Pharmaceutical Dosage Form on Primesep B Column
August 7, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Labetalol on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Labetalol
Labetalol is primarily used for the management of hypertension. It is classified as a beta-blocker, which works by blocking the effects of endogenous chemicals such as epinephrine on the heart and blood vessels. Through this mechanism, a reduction in heart rate, blood pressure, and myocardial strain is achieved.
Labetalol can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 230 nm.
You can find detailed UV spectra of Labetalol and information about its various lambda maxima by visiting the following link.
| Column | Primesep B, 4.6 x 250 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 15% |
| Buffer | H2SO4 -0.2% |
| Flow Rate | 0.7 ml/min |
| Detection | UV 230 nm |
| Class of Compounds | beta-blocker |
| Analyzing Compounds | Labetalol |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
UV-Vis Spectrum of Labetalol
August 6, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
Separation of Labetalol on Newcrom R1 HPLC column
February 16, 2018
Labetalol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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