HPLC Method for Analysis of Labetalol (stereoisomers) in Pharmaceutical Dosage Form on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Labetalol on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Labetalol on Primesep B Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Labetalol

Labetalol is primarily used for the management of hypertension. It is classified as a beta-blocker, which works by blocking the effects of endogenous chemicals such as epinephrine on the heart and blood vessels. Through this mechanism, a reduction in heart rate, blood pressure, and myocardial strain is achieved.

Labetalol can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 230 nm.

You can find detailed UV spectra of Labetalol and information about its various lambda maxima by visiting the following link.

ColumnPrimesep B, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseMeCN – 15%
BufferH2SO4 -0.2%
Flow Rate0.7 ml/min
DetectionUV 230 nm
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
beta-blocker
Analyzing CompoundsLabetalol

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Labetalol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.