HPLC MS Method for Analysis of Labetalol in Pharmaceutical Dosage Form on Primesep 200 Column

HPLC Method for Labetalol on Primesep 200 by SIELC Technologies

HPLC Method for Analysis of Labetalol on Primesep 200 Column by SIELC Technologies

Labetalol is a medication with the chemical formula C19H24N2O3. It isprimarily used for the management of hypertension. It is classified as a beta-blocker, which works by blocking the effects of endogenous chemicals such as epinephrine on the heart and blood vessels. Through this mechanism, a reduction in heart rate, blood pressure, and myocardial strain is achieved. You can find detailed UV spectra of Labetalol and information about its various lambda maxima by visiting the following link

Labetalol can be retained, and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Formic Acid as a buffer. Detection is achieved using UV at 300 nm

ColumnPrimesep 200, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%
BufferFormic Acid – 0.2%
Flow Rate0.2 ml/min
DetectionUV 300 nm, SIM + 329

 

Class of Compounds
Beta-blocker
Analyzing CompoundsLabetalol

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Labetalol
Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.