HPLC Method for Abacavir on Primesep 100 by SIELC Technologies
Abacavir, often written as ABC, is a medication with the formula C14H18N6O. It is used primarily as a part of HIV/AIDS treatments. As a nucleoside reverse transcriptase inhibitor and a guanosine, it works through inhibiting viral replication. It is in the World Health Organization’s List of Essential Medicines. You can find detailed UV spectra of Abacavir and information about its various lambda maxima by visiting the following link.
Abacavir can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is carried out using LC MS
| Column | Primesep 100, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN/H2O – 60/40 to 80/20% over 10 mi |
| Buffer | Ammonium formate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | LC MS ESI SIM 287 (M+H)+ , UV 296 nm |
| Class of Compounds | Medication |
| Analyzing Compounds | Abacavir |
Application Column
Primesep 100
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
LC MS Detection
