HPLC Method for Analysis of Pyridine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyridine on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Pyridine on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyridine

Pyridine is an aromatic heterocyclic organic compound with the chemical formula C₅H₅N. It is structurally related to benzene, with one methine group (=CH−) replaced by a nitrogen atom.

Pyridine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 250 nm.

You can find detailed UV spectra of Pyridine and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 70%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 250 nm
Limit of Detection5 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Heterocyclic aromatic
Analyzing CompoundsPyridine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Pyridine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.