HPLC Method for Separation PEI and PVP on PVP Column

 

HPLC Method for separation PVP, Polyethylenimine PEI on PVP by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of PVP, Polyethylenimine PEI

PEI: Polyethylenimine

  • Structure: Branched or linear polymer made of ethylene imine units.
  • Formula: (C2H5N)n(C_2H_5N)_n(C2​H5​N)n​
  • Charge: Cationic (positively charged in aqueous solution)
  • Properties:
    • Highly branched (usually) with a high density of amine groups.
    • Strong polycation—used to bind negatively charged molecules like DNA or proteins.
  • Applications:
    • Gene delivery (non-viral vectors)
    • Flocculation in water treatment
    • Adhesion promoter
    • Antimicrobial coatings
    • Paper and textile processing

🧪 PVP: Polyvinylpyrrolidone

  • Structure: Linear polymer of N-vinylpyrrolidone
  • Formula: (C6H9NO)n(C_6H9NO)_n(C6​H9NO)n​
  • Charge: Nonionic
  • Properties:
    • Water-soluble
    • Biocompatible and inert
    • Forms clear solutions
  • Applications:
    • Pharmaceutical excipient (binder in tablets, stabilizer in suspensions)
    • Cosmetic formulations (e.g., hair sprays, lotions)
    • Blood plasma volume expander (historically)
    • Adhesives, coatings, inks

PVP, Polyethylenimine PEI can be retained and analyzed using the PVP column. The analysis utilizes an step gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate. Detection is performed using ELSD.

Column PVP, 2.1 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase Step gradient MeCN/H2O
Buffer Ammonium Acetate  pH 4.0 –  20 mM
Flow Rate 0.2 ml/min
Detection ELSD
LOD PVP 0.5 ppm, PEI 1.7 ppm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds Polymer
Analyzing Compounds PVP, Polyethylenimine PEI

Application Column

PVP

Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
PVP
Polyethylenimine PEI
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.