HPLC Method for Determination of Azithromycin on Primesep 200 Column

HPLC Method for Azithromycin on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin.

Azithromycin is an antibiotic with the chemical formula C38H72N2O12. It is used in the treatment of various bacterial infections including but not limited to pneumonia, strep throat, and STI.  As a medication, it is available in a variety of forms: tablets, capsules, injections, and solutions. It is on the WHOs list of essential medicines and is classified as critically important.

In reversed-phase HPLC, it generally exhibits significant tailing.  The tailing can be substantially reduced by using a mixed-mode Primesep 200 column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.

Condition
Column Primesep 200, 2.1 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 75%
Buffer Ammonium Formate pH 3.0 – 50 mM
Flow Rate 0.2 ml/min
Detection ELSD, 50C

 

Description
Class of Compounds Drug
Analyzing Compounds Azithromycin

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Azithromycin
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.