Azithromycin

Azithromycin structural formula

CAS Number83905-01-5
Molecular FormulaC38H72N2O12
Molecular Weight748.996
InChI KeyMQTOSJVFKKJCRP-BICOPXKESA-N
LogP4.02
Synonyms
  • Azithromycin
  • (2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-2-Ethyl-3,4,10-trihydroxy-3,5,6,8,10,12,14-heptamethyl-15-oxo-11-{[3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranosyl]oxy}-1-oxa-6-azacyclopentadecan-13-yl 2,6-dideoxy-3-C-methyl-3-O-methyl-alpha-L-ribo-hexopyranoside
  • 1-Oxa-6-azacyclopentadecan-15-one, 13-[(2,6-dideoxy-3-C-methyl-3-O-methyl-alpha-L-ribo-hexopyranosyl)oxy]-2-ethyl-3,4,10-trihydroxy-3,5,6,8,10,12,14-heptamethyl-11-[[3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranosyl]oxy]-, (2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-
  • 83905-01-5
  • AZM
  • 9-Deoxo-9a-methyl-9a-aza-9a-homoetrythromycin A
  • Aruzilina
  • Arzomicin
  • Azadose
  • Azatril
  • Aziromycin
  • Azisara
  • Azithral
  • Azithromycin A
  • Azitrocin
  • Azitromax
  • Azomycin
  • Hemomycin
  • Macrozit
  • N-Methyl-11-aza-10-deoxo-10-dihydroerythromycin A
  • Sumamed
  • Sumazid
  • Trozocina
  • Ultreon
  • Xithron
  • Xithrone
  • Zi-Factor
  • Zithromac
  • Zithromax
  • Zithromax IV
  • Zitromax
  • Zythromax
  • BRN 5387583
  • Misultina
  • Mixoterin
  • Zithrax
  • Zitrotek
  • Azasite
  • Z-Pak
  • Azitromicine
  • Aritromicina
  • Azithromycine
  • Azithromycinum
  • UNII-J2KLZ20U1M
  • Trulimax
  • (2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)13-((2,6-Dideoxy-3-C-methyl-3-O-methyl-alpha-L-ribo-hexopyranosyl)oxy)-2-ethyl-3,4,10-trihydroxy-3,5,6,8,10,12,14-heptamethyl-11-((3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranosyl)oxy)-1-oxa-6-azacyclopentadecan-15-oneAzithromycin
  • 104491-80-7
  • 142556-82-9

Applications:

HPLC Method for Determination of Azithromycin on Primesep 200 Column

September 2, 2022

HPLC Method for Azithromycin on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin.

Azithromycin is an antibiotic with the chemical formula C38H72N2O12. It is used in the treatment of various bacterial infections including but not limited to pneumonia, strep throat, and STI.  As a medication, it is available in a variety of forms: tablets, capsules, injections, and solutions. It is on the WHOs list of essential medicines and is classified as critically important.

In reversed-phase HPLC, it generally exhibits significant tailing.  The tailing can be substantially reduced by using a mixed-mode Primesep 200 column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.

Condition
Column Primesep 200, 2.1 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 75%
Buffer Ammonium Formate pH 3.0 – 50 mM
Flow Rate 0.2 ml/min
Detection ELSD, 50C

 

Description
Class of Compounds Drug
Analyzing Compounds Azithromycin

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Azithromycin
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Azithromycin on Primesep SB Column

September 9, 2020

HPLC Method for Azithromycin on Primesep SB by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin.

Azithromycin is an antibiotic used in the treatment of various bacterial infections.  It is on the WHOs list of essential medicines and is classified as critically important.  In reversed-phase HPLC, it generally exhibits significant tailing.  The tailing can be substantially reduced by using a mixed-mode Primesep SB column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.

Condition
Column Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 15/85%
Buffer AmFm pH 3.0 – 50 mM
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
 Drug, Antibiotics
Analyzing Compounds Azithromycin

 

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Azithromycin
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Azithromycin on Newcrom R1 HPLC column

May 16, 2018
Separation of Azithromycin on Newcrom R1 HPLC column

Azithromycin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Azithromycin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.