| CAS Number | 83905-01-5 |
|---|---|
| Molecular Formula | C38H72N2O12 |
| Molecular Weight | 748.996 |
| InChI Key | MQTOSJVFKKJCRP-BICOPXKESA-N |
| LogP | 4.02 |
| Synonyms |
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Applications:
HPLC Method for Determination of Azithromycin on Primesep 200 Column
September 2, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin
Azithromycin is an antibiotic used in the treatment of various bacterial infections. It is on the WHOs list of essential medicines and is classified as critically important. In reversed-phase HPLC, it generally exhibits significant tailing. The tailing can be substantially reduced by using a mixed-mode Primesep 200 column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
| Column | Primesep 200, 2.1×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 75% |
| Buffer | Ammonium Formate pH 3.0 – 50 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, 50C |
| Class of Compounds | Drug |
| Analyzing Compounds | Azithromycin |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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HPLC Determination of Azithromycin on Primesep SB Column
September 9, 2020
Azithromycin is an antibiotic used in the treatment of various bacterial infections. It is on the WHOs list of essential medicines and is classified as critically important. In reversed-phase HPLC, it generally exhibits significant tailing. The tailing can be substantially reduced by using a mixed-mode Primesep SB column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.
| Column | Primesep SB, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 15/85% |
| Buffer | AmFm pH 3.0 – 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Drug, Antibiotics |
| Analyzing Compounds | Azithromycin |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Separation of Azithromycin on Newcrom R1 HPLC column
May 16, 2018
Azithromycin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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