HPLC Method for Determination of Azithromycin on Primesep 200 Column

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Azithromycin

 

Azithromycin is an antibiotic used in the treatment of various bacterial infections.  It is on the WHOs list of essential medicines and is classified as critically important.  In reversed-phase HPLC, it generally exhibits significant tailing.  The tailing can be substantially reduced by using a mixed-mode Primesep 200 column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.

Condition
Column Primesep 200, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeCN – 75%
Buffer Ammonium Formate pH 3.0 – 50 mM
Flow Rate 0.2 ml/min
Detection ELSD, 50C

 

Description
Class of Compounds Drug
Analyzing Compounds Azithromycin

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Azithromycin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.