HPLC Determination of Azithromycin on Primesep SB Column

HPLC.cloud View on hplc.cloud Azithromycin is an antibiotic used in the treatment of various bacterial infections.  It is on the WHOs list of essential medicines and is classified as critically important.  In reversed-phase HPLC, it generally exhibits significant tailing.  The tailing can be substantially reduced by using a mixed-mode Primesep SB column with a simple MS-compatible mobile phase of water, acetonitrile (ACN) and ammonium formate (AmFm) buffer.

Condition

Column Primesep SB, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 15/85%
Buffer AmFm pH 3.0 - 50 mM
Flow Rate 1.0 ml/min
Detection CAD
 

Description

Class of Compounds Drug, Antibiotics
Analyzing Compounds Azithromycin
 
Application Analytes:
Azithromycin