HPLC Method for Analysis of Citrulline on Primesep 100 Column

HPLC Method for Citrulline on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Citrulline.

Citrulline is an alpha-amino acid with the chemical formula C6H13N3O3. It is a key intermediate in the urea cycle. It can be derived from a variety of sources, such as arginine, ornithine, and asymmetric dimethylarginine. Commercially, it is used as a dietary supplemant as L-citrulline boots the production of nitric oxide, which helps with arterial function.

Citrulline can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseH2SO4 – 0.1%
BufferMeCN/H2O – 10/90%
Flow Rate1.0 mL/min
DetectionUV 200 nm
LOD*0.3 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsAmino Acid
Analyzing CompoundsCitrulline

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Citrulline
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.