High Performance Liquid Chromatography (HPLC) Method for Analysis of Aniline on Primesep 100 by SIELC Technologies

Aniline is an organic compound with the chemical formula C₆H₇N. It consists of a phenyl group attached to an amino group. Aniline is a primary aromatic amine. It is a colorless to slightly yellow liquid with a characteristic odor. It is produced by the reduction of nitrobenzene or by the catalytic hydrogenation of nitrobenzene. Aniline is a precursor to many industrial chemicals. It is used in the manufacture of polyurethane foam, rubber chemicals, dyes, agricultural chemicals, and pharmaceuticals. Aniline derivatives are also used as intermediates in the production of pigments and perfumes. It is toxic and can be absorbed through the skin, inhaled, or ingested. It can cause methemoglobinemia, a condition where hemoglobin is converted to methemoglobin, which cannot carry oxygen efficiently. You can find detailed UV spectra of Aniline and information about its various lambda maxima by visiting the following link.

Aniline can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm