HPLC Method for Vitamin E, Vitamin E Acetate on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Vitamin E, Vitamin E Acetate
(±)-α-Tocopherol is a form of vitamin E, a fat-soluble antioxidant that protects cell membranes from oxidative damage by neutralizing free radicals. Widely found in dietary sources like nuts and vegetable oils, it plays a critical role in maintaining skin health, immune function, and overall cellular protection. α-Tocopheryl acetate, on the other hand, is a stable esterified form of α-tocopherol. Commonly used in dietary supplements and skincare products, it is converted to active α-tocopherol in the body, offering a prolonged shelf life and enhanced stability in formulations.
Vitamin E, Vitamin E Acetate can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ammonium formate and formic acid as a buffer. Detection is achieved using UV 292 nm or MS.
| Column | Lipak, 3.2 x 150 mm, 3 µm, 100 A |
| Mobile Phase | Gradient MeOH/H2O – 90/10 – 100/0% in 10 min, 5 min hold |
| Buffer | AmFm– 10 mM, FA – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 292 nm ESI MS SIM 431 +, 473+ |
| Class of Compounds | Fat Soluble Vitamins |
| Analyzing Compounds | Vitamin E, Vitamin E Acetate |
Application Column
Vitamin E Acetate
LC MS Detection
