HPLC UV Method for Analysis of Procarbazine Hydrochloride on Primesep 100 Column

HPLC Method for Procarbazine hydrochloride, Procarbazine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Procarbazine hydrochloride, Procarbazine on Primesep 100 Column by SIELC Technologies

Procarbazine is a chemotherapy medication with the formula C12H19N3O. It is used primarily in treatment of Hodgkin lymphoma and brain cancers. Itison the World Health Organization’s List of Essential Medicines. In treatment of Hodgkin’s lymphoma, it is typically delivered as a part of the BEACOPP regiment, being the last “P” in the acronym. It is used in other combinations, usually as a part of PCV, when treating brain cancer. You can find detailed UV spectra of Procarbazine hydrochloride, Procarbazine and information about its various lambda maxima by visiting the following link.

Procarbazine hydrochloride, Procarbazine can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and H2SO4 as a buffer. Detection is carried out using UV

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50%
BufferH2SO4  – 0.1%
Flow Rate1 ml/min
DetectionUV 230 nm
LOD*6 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of CompoundsMedication
Analyzing CompoundsProcarbazine hydrochloride, Procarbazine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Procarbazine
Procarbazine hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.