HPLC Method for Analysis of Tetracycline on Primesep 100 Column on Alltesta™

HPLC Method for Tetracycline on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetracycline

Tetracycline is an antibiotic with the molecular formula C22H24N2O8. It is a bacteriostatic agent, therefore it prevents bacteria from growing and multiplying through inhibiting protein synthesis within bacteria. It is used to treat a variety of infections.

Tetracycline can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 20-80%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsMedication
Analyzing CompoundsTetracycline

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Tetracycline
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.