HPLC Method for Analysis of Pseudoephedrine on Primesep 100 Column

HPLC Method for Pseudoephedrine (PSE) on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pseudoephedrine (PSE).

Pseudoephedrine is one of the most popular decongestant drugs on the market, in part due to its efficacy and in part due to its status as an amphetamine.  It has the chemical formula C10H15NO.  It has been reported to have been misused as a psychostimulant, since it is accessible over-the-counter.  It was a banned substance by the International Olympic Committee (IOC) until 2004, but was banned again in 2010.

Pseudoephedrine (PSE) can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 60%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 200 nm

 

Description
Class of Compounds Drug
Analyzing Compounds Pseudoephedrine (PSE)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Pseudoephedrine (PSE)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.