HPLC Method for Analysis of Phthalic acid on Primesep 100 Column on Alltesta™

HPLC Method for Phthalic Acid on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Phthalic Acid

Phthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂. It consists of a benzene ring with two carboxylic acid groups (-COOH) attached to adjacent carbon atoms (in the ortho position). It is mainly produced by the oxidation of naphthalene or o-xylene. In the form of phthalic anhydride, it is used as an industrial chemical. It is primarily used to make phthalates, which are esters of phthalic acid.

Phthalic Acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsAcid
Analyzing CompoundsPhthalic Acid

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Phthalic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.