HPLC Method for Analysis of Secnidazole, Tinidazole, Metronidazole on Newcrom AH and Newcrom R1 Column by SIELC Technologies
Tinidazole is a medication with the chemical formula C8H13N3O4S. It is used primarily to treat infections caused by anaerobic bacteria and protozoa. It has a longer half-life than other medications of a similar class. You can find detailed UV spectra of Tinidazole and information about its various lambda maxima by visiting the following link.
Metronidazole is an antibiotic with C6H9N3O3 molecular formula. It works strongly against anaerobic bacteria and protozoa. It is available as oral tablets, capsules, topical gels, and injections. To truly kill bacteria and prevent infection, a full course of the medication must be completed as prescribed by a doctor. It is the most commonly prescribed among the three and is used to treat a wide range of infections. You can find detailed UV spectra of Metronidazole and information about its various lambda maxima by visiting the following link.
Secnidazole is a newer nitroimidazole antibiotic with a longer half-life than metronidazole, which allows for a shorter treatment duration. It is a nitroimidazole anti-infective drug with the chemical formula C7H11N3O3. It is used primarily to treat bacterial vaginosis and trichomoniasis. You can find detailed UV spectra of Secnidazole and information about its various lambda maxima by visiting the following link.
It is important to note that these antibiotics can have side effects such as nausea, vomiting, and diarrhea, and should only be used as directed by a healthcare professional. Additionally, alcohol should be avoided while taking these medications as they can cause a severe reaction.
Secnidazole, Tinidazole, Metronidazole can be separated and analyzed on a Newcrom AH (mixed-mode) and Newcrom R1 (reverse-phase) columns using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN, ACN), and phosphoric acid buffer. The analysis method can be UV detected at 270 nm with high resolution and peak symmetry.
Condition
| Column | Newcrom AH, 3.2 x 100 mm, 5 µm, 100 A, dual ended, Newcrom R1 |
| Mobile Phase | MeCN – 20% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 270 nm |
Description
| Class of Compounds | Nitroimidazole antibiotics |
| Analyzing Compounds | Secnidazole, Tinidazole, Metronidazole |
Application Column
Newcrom AH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Secnidazole
Tinidazole
