HPLC Method for Analysis of Neopterin on Primesep 100 Column on Alltesta™

HPLC Method for Neopterin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Neopterin 

Neopterin is an pteridine with the chemical formula C9H11N5O4. It is indicative of a pro-inflammatory immune status, therefore it typically serves as a marker of the activation of the cellular immune system.

Neopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 5%
BufferAmmonium Formate
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsOrganic Compound
Analyzing CompoundsNeopterin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Neopterin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.