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CAS Number | 2009-64-5 |
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Molecular Formula | C9H11N5O4 |
Molecular Weight | 253.22 |
InChI Key | BMQYVXCPAOLZOK-XINAWCOVSA-N |
LogP | -1.5 |
Synonyms |
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Applications:
HPLC Method for Separation of Biopterin, Rhamnopterin, Neopterin, Folic Acid and Aminopterin on Primesep 100 Column
June 8, 2023
HPLC Method for Analysis of Biopterin, Rhamnopterin, Neopterin, Folic Acid, Aminopterin on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
These compounds, including Biopterin, Rhamnopterin, Neopterin, Folic Acid, and Aminopterin, are all related to the chemical structure known as pterin. Pterins are a group of heterocyclic compounds composed of a pteridine ring system, which consists of two fused six-membered rings, a pyrimidine ring and a pyrazine ring. Here’s a brief overview of each:
- Biopterin (or Tetrahydrobiopterin): This is a coenzyme in the synthesis of several neurotransmitters, including dopamine, norepinephrine, and serotonin. It also plays a role in the production of nitric oxide. Deficiencies in the enzyme that recycles tetrahydrobiopterin can lead to phenylketonuria (PKU), a metabolic disorder.
- Rhamnopterin:
- Neopterin: This pteridine derivative is produced by the human immune system upon activation by interferon-gamma and serves as a marker for immune system activation. Elevated levels can be found in various disorders, such as viral infections, autoimmune diseases, or malignancies.
- Folic Acid: Also known as vitamin B9, folic acid is crucial for many functions in the body, including DNA synthesis and repair, cell division, and growth. Folic acid must be obtained through diet or supplementation, as humans cannot synthesize it.
- Aminopterin: An antifolate (folic acid antagonist), aminopterin was one of the first chemotherapy drugs used to treat cancer. It works by inhibiting dihydrofolate reductase, an enzyme that converts folic acid to its active form, thereby interfering with cell growth and division. Aminopterin is highly toxic and has been largely replaced by less toxic and more effective drugs, such as methotrexate.
- It’s important to note that while these compounds all contain a pteridine ring, they have very different biological activities and uses. Always consult with a healthcare provider or relevant professionals for accurate information.
These five pterins can be retained, separated and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 275 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin, Rhamnopterin, Neopterin, Folic Acid, Aminopterin
Condition
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
Mobile Phase | Gradient MeCN -5-60%, 10 min |
Buffer | Ammonium formate pH 3.0 – 50 mM |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm |
Peak Retention Time | 2.21, 3.28, 3.81, 6.85, 11.21 min |
Sample concentration | 0.02 mg/ml |
Injection volume | 5 µl |
Sample diluent | H2O + NaOH |
LOD | 10 ppb |
Description
Class of Compounds | Pteridines |
Analyzing Compounds | Biopterin, Rhamnopterin, Neopterin, Folic Acid, Aminopterin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Biopterin
Folic Acid
Neopterin
Rhamnopterin
HPLC Method for Separation of Biopterin, Rhamnopterin and Neopterin on Primesep 100 Column
June 7, 2023
HPLC Method for Analysis of Biopterin, Rhamnopterin, Neopterin on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
- Biopterin: This is a coenzyme involved in the biosynthesis of several important bioactive substances, including neurotransmitters like serotonin and dopamine. It’s also integral to the creation of nitric oxide, a potent vasodilator.
- Rhamnopterin is a derivative of biopterin, demonstrating remarkable properties in dietary studies. Research has shown that when it is administered alongside certain carcinogens, rhamnopterin can decrease the occurrence of liver tumors in rats. Additionally, it serves as an internal standard for measuring biopterin and neopterin concentrations in rat plasma.
- Neopterin: This is a catabolic product of guanosine triphosphate (GTP), a molecule involved in energy transfer. It’s often used as a marker for immune system activation, particularly in response to certain diseases, including viral infections, autoimmune diseases, and cancer.
These three pterins can be retained, separated and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 275 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin, Rhamnopterin, Neopterin
Condition
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
Mobile Phase | MeCN/H2O -5/95% |
Buffer | Ammonium formate pH 3.0 – 20 mM |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm |
Peak Retention Time | 3.28, 4.01, 4.85 min |
Sample concentration | 0.02 mg/ml |
Injection volume | 5 µl |
Sample diluent | H2O + NaOH |
LOD | 20 ppb |
Description
Class of Compounds | Pteridines |
Analyzing Compounds | Biopterin, Rhamnopterin, Neopterin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Neopterin
Rhamnopterin
HPLC Method for Analysis of Neopterin on Primesep 100 Column
June 7, 2023
HPLC Method for Analysis of Neopterin on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Biopterin belongs to the chemical group known as pteridines. Pteridines are aromatic compounds that consist of a pyrimidine ring fused to a pyrazine ring. These compounds are involved in various biological processes and can be found in different organisms, including bacteria, plants, and animals.
Biopterin, specifically, plays a critical role in the body as a cofactor in the synthesis of several important neurotransmitters, including serotonin, melatonin, dopamine, norepinephrine, and epinephrine. These neurotransmitters are crucial for many physiological functions, including mood regulation, sleep, and response to stress.
Deficiency in biopterin metabolism can lead to various disorders, such as neurodegeneration due to BH4 deficiency, a group of rare inherited neuro-metabolic disorders. This is why understanding and potentially modulating biopterin and its derivatives could be significant for certain therapeutic approaches.
Biopterin retained and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 275 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Neopterin
Condition
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
Mobile Phase | MeCN/H2O -5/95% |
Buffer | Ammonium formate pH 3.0 – 20 mM |
Flow Rate | 1.0 ml/min |
Detection | UV 275 nm |
Peak Retention Time | 3.28 min |
Sample concentration | 0.4 mg/ml |
Injection volume | 1 µl |
Sample diluent | H2O + NaOH |
LOD | 10 ppb |
Description
Class of Compounds | Pteridines |
Analyzing Compounds | Neopterin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A