Rhamnopterin

HPLC Method for Analysis of Biopterin, Rhamnopterin, Neopterin, Folic Acid, Aminopterin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

These compounds, including Biopterin, Rhamnopterin, Neopterin, Folic Acid, and Aminopterin, are all related to the chemical structure known as pterin. Pterins are a group of heterocyclic compounds composed of a pteridine ring system, which consists of two fused six-membered rings, a pyrimidine ring and a pyrazine ring. Here’s a brief overview of each:

1. Biopterin (or Tetrahydrobiopterin): This is a coenzyme in the synthesis of several neurotransmitters, including dopamine, norepinephrine, and serotonin. It also plays a role in the production of nitric oxide. Deficiencies in the enzyme that recycles tetrahydrobiopterin can lead to phenylketonuria (PKU), a metabolic disorder.
2. Rhamnopterin:
3. Neopterin: This pteridine derivative is produced by the human immune system upon activation by interferon-gamma and serves as a marker for immune system activation. Elevated levels can be found in various disorders, such as viral infections, autoimmune diseases, or malignancies.
4. Folic Acid: Also known as vitamin B9, folic acid is crucial for many functions in the body, including DNA synthesis and repair, cell division, and growth. Folic acid must be obtained through diet or supplementation, as humans cannot synthesize it.
5. Aminopterin: An antifolate (folic acid antagonist), aminopterin was one of the first chemotherapy drugs used to treat cancer. It works by inhibiting dihydrofolate reductase, an enzyme that converts folic acid to its active form, thereby interfering with cell growth and division. Aminopterin is highly toxic and has been largely replaced by less toxic and more effective drugs, such as methotrexate.
6. It’s important to note that while these compounds all contain a pteridine ring, they have very different biological activities and uses. Always consult with a healthcare provider or relevant professionals for accurate information.

These five pterins can be retained, separated and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 275 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin, Rhamnopterin, Neopterin, Folic Acid, Aminopterin

Condition

Description

HPLC Method for Analysis of Biopterin, Rhamnopterin, Neopterin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

1. Biopterin: This is a coenzyme involved in the biosynthesis of several important bioactive substances, including neurotransmitters like serotonin and dopamine. It’s also integral to the creation of nitric oxide, a potent vasodilator.
2. Rhamnopterin is a derivative of biopterin, demonstrating remarkable properties in dietary studies. Research has shown that when it is administered alongside certain carcinogens, rhamnopterin can decrease the occurrence of liver tumors in rats. Additionally, it serves as an internal standard for measuring biopterin and neopterin concentrations in rat plasma.
3. Neopterin: This is a catabolic product of guanosine triphosphate (GTP), a molecule involved in energy transfer. It’s often used as a marker for immune system activation, particularly in response to certain diseases, including viral infections, autoimmune diseases, and cancer.

These three pterins can be retained, separated and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 275 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin, Rhamnopterin, Neopterin

Condition

Description

HPLC Method for Analysis of Rhamnopterin on (Column not found) by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

Rhamnopterin, a derivative of L-biopterin, D-biopterin, and the oxidized form of tetrahydro-L-biopterin (BH4), has exhibited significant properties in dietary studies. When administered in a 0.5% concentration alongside the carcinogen 4-dimethylaminoazobenzene, it can reduce the incidence of liver tumors induced by this carcinogen in rats. In addition to these therapeutic applications, rhamnopterin has also been utilized as an internal standard for quantifying biopterin and neopterin levels in rat plasma, as demonstrated in LC-MS-based studies

Rhamnopterin retained and analyzed using a reverse-phase (Column variation not found) column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 275 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Rhamnopterin

Condition

Description