HPLC Method for Analysis of N-Nitrosodiethylamine on Newcrom R1 Column

HPLC Method for N-Nitrosodiethylamine on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of N-Nitrosodiethylamine 

N-Nitrosodiethylamine (NDEA) or Diethylnitrosamine is potential carcenogenic agent was found in some blood pressure medications. Those medications were recalled by FDA with the concern of high concentration of the chemical.
SIELC developed simple and fast HPLC method to measure presence of NDEA in drug formulations. This UV based method allows to detect ppb level of the compound in solution. Simple mobile phase comprised of acetonitrile (MeCN) and water with detection at 2** nm using Newcrom R1 column can be used to detect both the API and the NDEA.

ColumnNewcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferNo
Flow Rate1.0 ml/min
DetectionUV, 232 nm
Class of Compounds
Carcinogen, Zwitterionic, Hydrophobic
Analyzing CompoundsN-Nitrosodiethylamine

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
N-Nitrosodiethylamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.