HPLC Method for Analysis of Metronidazole on Newcrom R1 Column on Alltesta™

HPLC Method for Metronidazole on Newcrom R1 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Metronidazole

Metronidazole is an antibiotic with C6H9N3O3 molecular formula. It works strongly against anaerobic bacteria and protozoa. It is available as oral tablets, capsules, topical gels, and injections. To truly kill bacteria and prevent infection, a full course of the medication must be completed as prescribed by a doctor.

Metronidazole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

ColumnNewcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 5%
BufferH3PO4 – 0.21
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsAntibiotic
Analyzing CompoundsMetronidazole

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Metronidazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.