HPLC Method for Analysis of Metalaxyl on Newcrom B Column on Alltesta™

HPLC Method for Metalaxyl (UV) on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metalaxyl (UV) 

Metalaxyl, also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate,  is a systemic fungicide with the chemical formula C15H21NO4.  It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.

Metalaxyl (UV) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferAmmonium Formate
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsPesticide
Analyzing CompoundsMetalaxyl (UV)

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Metalaxyl (UV)
Application Detection:
UV Detection
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