HPLC Method For Analysis Of Meldonium in Mildronate Tablet Dosage Form on Primesep 100 Column

HPLC Method for Meldonium on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Meldonium.

Meldonium is a medication with the chemical formula C6H14N2O2.  It is a popular treatment for ischemia in Eastern Europe.  Due to it’s metabolic modulating effects as well as it altering enzymatic reaction in the body it is considered to be a performance-enhancing drug, therefore, it has been banned by the World Anti-Doping Agency since 2016.  It works as a fatty acid oxydation inhibitor by inhibiting enzymes in the carnitine biosynthesis pathways.

Meldonium can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 25/75%
Buffer HClO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV, 205 nm

 

Description
Class of Compounds
 Amino acid, Drug
Analyzing Compounds Meldonium

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Meldonium
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.