HPLC Method for Analysis of Lamivudine on Primesep 100 Column on Alltesta™

HPLC Method for Lamivudine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Lamivudine 

Lamivudine is an antiviral medication with the chemical formula C8H11N3O3S. It is used primarily to treat infections caused by the human immunodeficiency virus (HIV) and hepatitis B virus (HBV). It belongs to a class of drugs known as nucleoside reverse transcriptase inhibitors (NRTIs). You can find detailed UV spectra of Lamivudine and information about its various lambda maxima by visiting the following link.

Lamivudine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN -50/50%
BufferAmFm pH 3.0 – 20 mM
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsMedication
Analyzing CompoundsLamivudine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Lamivudine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.